Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
ABS 001-07-02
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
7
Immunogen
Recombinant form of human activated protein C.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_1070442
Product Specific Information
ABS 001-07-02 has specificity for a conformation-dependant neoepitope that is expressed in activated protein C upon complex-formation with alpha1-antitrypsin. No reaction is seen to non-complexed alpha1-antitrypsin and only very little cross reaction to protein C zymogen. Note that specificity is calcium dependent.
ABS 001-07-02 has been successfully used in ELISA procedures. ABS 001-07-02 reacts strongly with APC-AAT complexes in ELISA. It can be used in sandwich ELISA in combination with a polyclonal anti-protein C antiserum. Note, that the conformational neoepitope expressed in the complex can also be expressed in APC coated directly onto a high-binding microtiter plate.
The epitope specificity of ABS 001-07-02 is not determined.
NOTE: Concentration is lot-dependent and can vary from 0.85-1.15 mg/mL
Target Information
The neoepitope is conformation-dependent and expressed in activated protein C upon complex-formation with α1-antitrypsin. Protein C is a vitamin K-dependent serine protease produced in the liver and made up of 2 polypeptide chains. The 62 kDa proenzyme is activated by thrombin and the active enzyme cleaves factor Va and VIIIa and thus inhibits blood coagulation. The molecular weight of the active enzyme is 55 kDa and the normal concentrations in human plasma is approximately 1-3 ng/mL because of the very fast turnover, the proenzyme concentration is approximately 3 µg/mL. The activated protein C (APC) is inhibited by members of the serine protease inhibitor (serpin) family, of which α1-antitrypsin (AAT) and protein C inhibitor (PCI) are the most important.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: APC
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support